Phenylalanine Malonate Broth (Shaw and Clarke Medium) (DM606) is recommended for differentiation of members of Enterobacteriaceae on the basis of their ability to utilize malonate and produce pyruvic acid from phenylalanine.
Ingredients | Gms / Liter |
Yeast extract | 1.00 |
Sodium malonate | 3.00 |
DL-Phenylalanine | 2.00 |
Ammonium sulphate | 2.00 |
Dipotassium phosphate | 0.60 |
Monopotassium phosphate | 0.40 |
Sodium chloride ` | 2.00 |
Bromo thymol blue | 0.025 |
Final pH: 6.3 ± 0.2 at 25°C | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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