Dehydrated Culture Media

Complete nourishment for microbial growth

  • Enriched with nutritive value
  • Maintained superior quality for several applications
  • Strictly manufactured under a stringent environment to comply with standards
  • Longer shelf life
Dehydrated Culture Media

Culturing cells in natural or artificial/synthetic media is one of the foremost techniques in life sciences. The cells are either cultured for isolation and identification purposes or for the production of secondary metabolites including antibiotics, antitumor agents, enzymes, pigments, siderophores, etc. 

Dehydrated culture media plays vital role in microbe culture cultivation and is extensively used in pharmaceuticals, hospitals, biotech industries, research institutes, etc. These media can be simple or complex. The appropriate concentration of various components is essentially required for in vitro cultivation.

DCM application includes checking microbial flora in any samples like tablets,milk,water,food,air etc alongwith identification of microbes at species level.

Micromaster offers various DCM like simple media, selective/differential media for the cultivation of microbes. We have formulated and developed DCM for complete microbe nourishment to accelerate their adaptation and persistent survival, healthy colony growth, and specific functions leading to easy and rapid detection

Our Expertise

Air-tight caps without inner

Avoids deterioration of the media powder. No inhalation of media while opening.

Ergonomic bottles

Easy to handle

Longer shelf-life

Maintenance of the stock.


It is a powdered form of nutritional components formulated for the growth of bacteria yeast and fungi.

Media is broadly classified into media broth, media agar, and semisolid media.

Basal media, Selective media, and enriched media.

Media is used to grow, isolate, identify and multiply microorganisms in a laboratory.

The culture media are designed to guarantee the viability of isolated microorganisms and are used for the enrichment of bacteria to acquire a sufficient amount of cells for further examination.

Microbiological culture media are defined according to ISO 11133-1 Guidelines and contain a large variety of ingredients to support the luxuriant cultivation of microorganisms:

    • Nutrients (e. g. Biological extracts, Digests of proteins)
    • Source of energy (e. g. Glucose)
    • Salts for maintaining adequate osmolarity (e.g. NaCl)
    • Enrichments (essential ingredients for auxotrophic or fastidious microorganisms)
    • Antioxidants and neutralizing agents against toxic substances like disinfectants (e. g. blood, coal, letheen)
    • Reducing agents to remove oxygen from media and maintain anaerobic or microaerophilic culture conditions
    • Indicator substances which indicate pH-shifts or cha nge of the redox state
    • Buffer for pH-regulation (e. g. potassium and sodium phosphates)
    • Chromogenic or fluorogenic nutrients, which are hydrolized by the microorganism and release a colored or flourescent hydrolysis product as indicator.
    • Selective agents to suppress the growth of unwanted microorganisms (e.g. chemicals, antibiotics, fungistatic agents)
    • Neutralizing agents for antibiotics or disinfectants (e.g. letheen, polysorbate) Solidifying agents (e.g. agar, gelatine) Water

Suitable production controls and sterilization conditions as well as a comprehensive quality control system are followed to assure reliable product stability.

Weigh the appropriate amount of media powder as per mentioned on the bottle and dissolve it in deionized water. Autoclave at 121 Degree Celsius for 20 min.

Sterility testing, bioburden testing, environmental monitoring program.






Storage of Dehydrated Culture Media:
The majority of Dehydrated Culture Media should be stored at a room temperature within the range of 10-30°C in a dry place, away from sources of heat and sunlight; a few may be stored at 2- 8°C.
Dehydrated culture media are hygroscopic. When bottles of dehydrated media opened for initial use should be tightly closed as soon as possible to protect them from hydration. The stock of dehydrated media should be rotated to ensure the fresh product is in use. The expired products should be discarded. The date of expiry applies to the products in their intact containers when stored as directed. Do not use a product if it fails to meet specifications for identity and performance. Prior to use, verify that the physical characteristics of the powder are typical. Hydration can lead to caking and/or microbial contamination which may render the properties of the culture medium making it unfit for use.

Storage of Ready-to-use media: Ready-to-use media should be stored under appropriate temperature as mentioned above and should be kept away from light.
Store the media in a position, in which the label can be easily read.
Avoid storage at temperatures below 2 °C, because it may freeze the agar in solid media. Even in refrigerators set at 4°C, some areas may have a lower temperature. Frozen products should be discarded. Water is the main ingredient of culture media. To reduce condensation in the plates and the plastic bags, a desiccant has been added. Any fluctuation of temperature during transport and storage should be avoided as this may lead condensation. The shelf life of the product is mentioned on the product label. Do not use expired culture media for microbiological examinations.

The expiry date mentioned on the label of Micromaster Dehydrated Culture Media refers to the shelf life of the product. It applies to the products in their intact containers when stored as directed. The total shelf life is typically 3-5 years.
Once opened, the total shelf life of Dehydrated Culture Media, Peptones and Hydrolysates is automatically reduced. The shelf life also depends on the frequency of exposure of media to atmospheric conditions.
Deterioration of particularly hygroscopic products may be observed within six months of opening. Dehydrated media should be examined carefully after several months of opening to ensure maintenance of physical integrity and should only be used if they still appear free-flowing. The media should be tested for performance and shelf life, using positive and negative microbiological control strains, after such extended periods of opening.
In order to maximise the shelf life of Dehydrated Culture Media, ensure that containers are not stored in areas of high humidity, e.g. autoclave areas.

Best use within one year after the date of manufacturing.

Store in dry and cool places, away from sunlight at temperatures as mentioned on the product label. The lid should be tightly closed for hygroscopic media.

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