Antibiotic Assay Medium No. 32 (DM005) is recommended for Bacillus subtilis innoculum preparation, during assay of Dihydrostreptomycin and Vancomycin.
| Ingredients | Gms / Liter |
| Peptic digest of animal tissue (Peptone) | 6.00 |
| Casein enzymic hydrolysate | 4.00 |
| Yeast extract | 3.00 |
| Beef extract | 1.50 |
| Dextrose | 1.00 |
| Manganese sulphate | 0.30 |
| Agar | 15.00 |
| Final pH: 6.6 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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