Hanahan’s Broth (SOB medium) (DM581) is recommended for cultivation of recombinant strains of Escherichia coli.
Ingredients | Gms / Liter |
Casein enzymic hydrolysate | 20.00 |
Yeast extract | 5.00 |
Sodium chloride | 0.50 |
Magnesium sulphate | 2.40 |
Potassium chloride | 0.186 |
Final pH: 7.0 ± 0.2 at 25°C | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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