Hemmes Medium Base (DM343) is recommended for biochemical differentiation of Salmonella and Shigella species based on dextrose, lactose, sucrose fermentation, motility, hydrogen sulphide, indole and urease production.
Ingredients | Gms / Liter |
Casein enzymic hydrolysate | 10.00 |
Yeast extract | 3.00 |
Dextrose | 0.30 |
Lactose | 10.00 |
Sucrose | 10.00 |
Sodium chloride | 4.00 |
Ferrous sulphate | 0.04 |
Sodium thiosulphate | 0.10 |
Phenol red | 0.015 |
Agar | 5.50 |
Final pH: 7.2 ± 0.2 at 25°C | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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