Fraser Broth Base (DM1292) is recommended for a primary and secondary enrichment medium for isolation and enumeration of Listeria monocytogenes from food and animal feeds.
| Ingredients | Gms / Liter |
| Peptic digest of animal tissue | 5.00 |
| Casein enzymic hydrolysate | 5.00 |
| Yeast extract | 5.00 |
| Meat extract | 5.00 |
| Sodium chloride | 20.00 |
| Disodium hydrogen phosphate.2H2O | 12.00 |
| Potassium dihydrogen phosphate | 1.35 |
| Esculin | 1.00 |
| Lithium chloride | 3.00 |
| Final pH: 7.2 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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