An inoculation loop, also called a sterile flexi loop or nichrome loop, is a simple tool used mainly by microbiologists to pick up and transfer a small sample (inoculum) from a culture of microorganisms having size of 2.2mm and 4mm and available in calibrated to 0.005ml and 0.01ml . Inoculation loop is made up of polystyrene material, gamma Irradiated easy to handle and dispose. Nichrome loops have some disadvantages such as, it required to cool down for the room temperature after flaming and then it can be used , so its time consuming and if nichrome loop not cool down properly then it may kill the bacterial from sample, from where we need to collect the sample and it may give us false negative reports. Disposable inoculating loops and needles eliminate the risk of infection because they do not need flaming and thus form the aerosol of pathogenic substances. improper sterilization is eliminated because of Cross-contamination.
Sterile Flexi Loop
Nutrient constituents for growth of microorganisms.
An inoculation loop, also called a sterile flexi loop or nichrome loop, is a simple tool used mainly by microbiologists to pick up and transfer a small sample (inoculum) from a culture of microorganisms having size of 2.2mm and 4mm and available in calibrated to 0.005ml and 0.01ml .
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It is a metal material made up of platinum, nickel and rhodium. It can fit into the loop holder and available in different sizes 1 – 4mm.
It is use for inoculation of culture, sample collection and in streaking of the plates
Streaking technique
It transfers the bacteria into the growth medium like flexiloops. It is easy to reuse. It can get sterile by Fleming. Because of nichrome wire property it resists the detoriation of loop during multiple cycle of Fleming.
Passing the nichrome loop on gas burner flame when the loop become orange from heat
Loop is calibrated and easy to sterilise by flaming process. collection, transfer and retrieving organisms is easy. It avoids the cross contamination
loops contain many bacteria of the environment like air bacteria to remove the all contaminants from wire we should sterilise it by flaming. So, we able to collect actual sample and inoculate into the medium so to get precise result.
nichrome wire loop(1-4mm) and flexiloops, masterloop with holder
the environment like air bacteria to remove the all contaminants from wire we should sterilise it by flaming. So, we able to collect actual sample and inoculate into the medium so to get precise result.
It is a tool to collect the sample or inoculum and transfer it into the growth medium
1.Single individual packing
2.pack of 100oc and 500pc is available
3.It’s is smooth and flexible
4.smooth loops allows easy streaking
5.white colour, polystyrene and rigid structure.
1.Collection of microbial cultures
2.Inoculation of cultures
3.Streaking cultures on RTU plates
4.Disposable easy to use
5.Rigid enough to penetrate the media on subsurface inoculation