Wayne Sulphatase Agar Base is used for biochemical differentiation of Mycobacteria based on their ability to produce aryl sulphatase.
Formula / Liter:
Ingredients | g / L |
Tryptone | 0.50 |
L-Asparagine | 1.00 |
Potassium dihydrogen phosphate | 1.00 |
Disodium hydrogen phosphate | 2.50 |
Ferric ammonium citrate | 0.05 |
Magnesium sulphate | 0.01 |
Calcium chloride | 0.0005 |
*Zinc sulphate | 0.0001 |
*Copper sulphate | 0.0001 |
Tripotassium phenolphthalein sulphate | 0.650 |
Agar | 15.00 |
Final pH (at 25°C) 7.0 ±0.2 | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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