Triple Sugar Iron Agar (Agar Medium M) (DM254E) is recommended for identification of gram-negative enteric bacilli based on their ability to ferment dextrose, lactose and sucrose and produce hydrogen sulphide in compliance with EP.
Ingredients | Gms / Litre |
Peptones (casein and Beef) | 20.00 |
Beef extract | 3.00 |
Yeast extract | 3.00 |
Lactose monohydrate | 10.00 |
Sucrose | 10.00 |
Glucose monohydrate | 1.00 |
Sodium chloride | 5.00 |
Ferric ammonium citrate | 0.30 |
Sodium thiosulphate | 0.30 |
Phenol red | 0.025 |
Agar | 12.00 |
Final pH : 7.4±0.2 at 25°C | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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