Triple Sugar Iron Agar (Agar Medium M) (DM254E) is recommended for identification of gram-negative enteric bacilli based on their ability to ferment dextrose, lactose and sucrose and produce hydrogen sulphide in compliance with EP.
| Ingredients | Gms / Litre |
| Peptones (casein and Beef) | 20.00 |
| Beef extract | 3.00 |
| Yeast extract | 3.00 |
| Lactose monohydrate | 10.00 |
| Sucrose | 10.00 |
| Glucose monohydrate | 1.00 |
| Sodium chloride | 5.00 |
| Ferric ammonium citrate | 0.30 |
| Sodium thiosulphate | 0.30 |
| Phenol red | 0.025 |
| Agar | 12.00 |
| Final pH : 7.4±0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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