Standard Nutrient Broth No.2 (DM622) is recommended for enrichment of less fastidious bacteria.
| Ingredients | Gms / Liter |
| Meat peptone | 4.30 |
| Casein enzymic hydrolysate | 4.30 |
| Sodium chloride | 6.40 |
| Agar | 13.00 |
| Final pH: 7.5 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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