SIM Medium is recommended for the differentiation of enteric bacteria on the basis of hydrogen sulphide production, indole production and motility.
| Ingredients | Gms / Liter |
| Beef extract | 3.00 |
| Peptic digest of animal tissue | 30.00 |
| Peptonized iron | 0.02 |
| Sodium thiosulphate | 0.025 |
| Agar | 3.00 |
| Final pH: 7.3 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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