Modified Duncan Strong (DS) Medium (DM614) is recommended for isolation and differentiation of Clostridium perfringens from other Clostridia from foods on the basis of raffinose fermentation.
| Ingredients | Gms / Liter |
| Proteose peptone | 15.00 |
| Yeast extract | 4.00 |
| Sodium thioglycollate | 1.00 |
| Disodium phosphate | 10.00 |
| Raffinose | 4.00 |
| Final pH: 7.8 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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