MacConkey agars are slightly selective and differential plating media mainly used for the detection and isolation of gram-negative organisms from clinical, dairy, food, water, pharmaceutical and industrial sources. MacConkey Agar is used for isolating and differentiating lactose fermenting from lactose-non-fermenting gram-negative enteric bacilli. MacConkey Agar Base is used with added carbohydrate in differentiating coliforms based on fermentation reactions. MacConkey Agar without Crystal Violet is used for isolating and differentiating enteric microorganisms while permitting growth of staphylococci and enterococci. The medium can be used also to separate Mycobacterium fortuitum and M. chelonae from other rapidly growing mycobacteria. MacConkey Agar without Crystal Violet or Salt and MacConkey Agar without Salt are used for isolating and differentiating gram-negative bacilli while suppressing the swarming of most Proteus species.
Ingredients | Gms / Litre |
Pancreatic digest of gelatin | 17.00 |
Casein enzymic hydrolysate | 1.50 |
Peptic digest of animal tissue | 1.50 |
Lactose | 10.00 |
Bile salts | 1.50 |
Sodium chloride | 5.00 |
Neutral red | 0.03 |
Crystal violet | 0.001 |
Agar | 15.00 |
Final pH: 7.1 ± 0.2 at 25°C | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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