Kohn Two Tube Medium No.1 Base (DM646) is recommended for identification of Enterobacteriaceae on the basis of dextrose and mannitol fermentation and urease production.
| Ingredients | Gms / Liter |
| Peptic digest of animal tissue | 15.00 |
| Beef extract | 2.00 |
| Yeast extract | 2.00 |
| Dextrose | 1.00 |
| Mannitol | 10.00 |
| Phenol red | 0.05 |
| Agar | 16.00 |
| Final pH: 7.2 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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