Kligler Iron Agar (DM127) is use for the differentiation of members of the Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to liberate sulfides.
Ingredients | Gms / Liter |
Peptic digest of animal tissue | 15.00 |
Beef extract | 3.00 |
Yeast extract | 3.00 |
Proteose peptone | 5.00 |
Lactose | 10.00 |
Dextrose | 1.00 |
Ferrous sulphate | 0.20 |
Sodium chloride | 5.00 |
Sodium thiosulphate | 0.30 |
Phenol red | 0.024 |
Agar | 15.00 |
Final pH ( at 25°C) 7.4 ± 0.2 | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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