ISP Medium No. 4 (Inorganic Salt Starch Agar) (DM777) is recommended for cultivation and characterization of Streptomyces species as per International Streptomyces Project.
Ingredients | Gms / Liter |
Starch, soluble | 10.00 |
Dipotassium phosphate | 1.00 |
Magnesium sulphate.heptahydrate | 1.00 |
Sodium chloride | 1.00 |
Ammonium sulphate | 2.00 |
Calcium carbonate | 2.00 |
Ferrous sulphate.heptahydrate | 0.001 |
Manganous chloride, 7H2O | 0.001 |
Zinc sulphate, 7H2O | 0.001 |
Agar | 20.00 |
Final pH: 7.2 ± 0.2 at 25°C | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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