Hugh Leifson Medium (DM370BS) is recommended for detecting aerobic and anaerobic breakdown of glucose as per British Standards.
| Ingredients | g / L |
| Peptic digest of animal tissue | 2.00 |
| Sodium chloride | 5.00 |
| Dipotassium phosphate | 0.30 |
| Glucose | 10.00 |
| Bromothymol blue | 0.05 |
| Agar | 2.00 |
| Final pH: 6.8 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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