Boric Acid Broth (DM336) is recommended for the detection and presumptive identification of Escherichia coli on the basis of this organism to grow at 43°C and form gas in the presence of boric acid.
Formula / Liter:
| Ingredients | g/ L |
| Proteose peptone | 10.00 |
| Dipotassium phosphate | 12.20 |
| Lactose | 5.00 |
| Potassium dihydrogen phosphate | 4.10 |
| Boric acid | 3.25 |
| Final pH: 7.0±0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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