B12 Inoculum Broth (DM029) is recommended for inoculum preparation of Lactobacillus leichmannii ATCC 7830, for Vitamin B12 microbiological assay.
Ingredients | Gms / Liter |
Proteose peptone | 7.50 |
Yeast extract | 7.50 |
Dextrose | 10.00 |
Monopotassium phosphate | 2.00 |
Tomato juice (from 100 ml) | 5.00 |
Polysorbate 80 | 0.10 |
Final pH: 6.8 ± 0.2 at 25°C | |
Formula may be adjusted and/or supplemented as required to meet performance specifications |
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