B12 Assay Agar (Using E. coli Mutant Culture) (Harrison et al. Medium) (DM528) is recommended for Vitamin B12 assay using Escherichia coli mutant 113-3 Davis ATCC 11105.
For microbiological assay of Vitamin B12, a complete dehydrated medium contains all essential nutritives except Vitamin B12 for the growth of E.coli mutant 113-3 Davis ATCC11105. Addition of B12 in specified increasing concentration gives a growth response, which can be measured with zone reader.
Final pH : 7.2 ± 0.2 at 25°C
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