Antibiotic assay medium No. 40 (DM211U) is used in microbiological assay of Thiostrepton using Enterococcus hirae as test organism in accordance with United States Pharmacopoeia
| Ingredients | Gms / Liter |
| Yeast extract | 20.00 |
| Polypetone | 5.00 |
| Dextrose | 10.00 |
| Monobasic potassium phosphate | 2.00 |
| Polysorbate 80 | 0.10 |
| Agar | 10.00 |
| Final pH: 6.7 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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