Acetate Differential Agar (DM421) is recommended for the differentiation of Shigella species from other members of the genus Escherichia.
| Ingredients | Gms / Liter |
| Sodium acetate | 2.00 |
| Magnesium sulphate | 0.10 |
| Sodium chloride | 5.00 |
| Monoammonium phosphate | 1.00 |
| Dipotassium phosphate | 1.00 |
| Bromothymol blue | 0.08 |
| Agar | 20.00 |
| Final pH: 6.7 ± 0.2 at 25°C | |
| Formula may be adjusted and/or supplemented as required to meet performance specifications | |
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